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恒遠產(chǎn)品文獻:科研人血清引用文獻
發(fā)布時間:2021/1/28 17:03:41 閱讀次數(shù):992

【文獻標題】Evidence-based antioxidant activity of the essential oil from Fructus A. zerumbet on cultured human umbilical vein endothelial cells’ injury induced by ox-LDL

【作者】Xiang-chun Shen,Ling Tao,Wan-kui Li,et.al

【作者單位】貴陽醫(yī)學院(Guiyang Medical University)

【文獻中引用產(chǎn)品】

科研人血清

【關(guān)鍵詞】Essential oil, Fructus Alpiniae zerumbet, Human umbilical vein endothelial cells, Oxidized low-density lipoprotein, Oxidative stress

【DOI】doi:10.1186/1472-6882-12-174

【影響因子(IF)】1.9

出版期刊】《BMC Complementary Medicine and Therapies》

【產(chǎn)品原文引用】

Methods

Chemical and herbal materials

The culture medium was used M199 with Earle’s salts (Sigma-Aldrich), containing 20% (v/v) human serum (Shanghai Hengyuan, Shanghai, China), 5 U/ml penicillin G, 5 pg/ml streptomycin sulfate and 150 pg/ml endothelial cell growth supplement (Sigma-Aldrich).Cells reached confluency within approximately 4 d, and were cultured at 37°C in a humidified atmosphere with 5% CO2. The medium was changed a day after seeding and every 2 d thereafter.


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