【文獻(xiàn)標(biāo)題】CP‐31398 inhibits the progression of cervical cancer through reversing the epithelial mesenchymal transition via the downregulation of PAX2s
【作者】Ling Liu,Tan‐Tan Yu,Chen‐Chen Ren,et.al
【作者單位】鄭州大學(xué)第三附屬醫(yī)院（The Third Affiliated Hospital of Zhengzhou University）
The tumor tissues were selected, dehydrated using gradient alcohol,and soaked in 3% H2O2 for 10 min. After a wash under distilled water and antigen repair, the tissues were boiled in the 0.01 M citric acid buffer solution (pH = 6.0) at 95°C for 15–20 min. After natural cooling for 20 min to room temperature, the tissues were washed by PBS three times, each time for 5 min. Then 100 μl 5% BSA blocking liquid was added and incubated at 37°C for 30 min. Then the diluted
primary rabbit monoclonal antibodies, including E‐cadherin (ab40772, 1:500; Abcam Inc.), vimentin (ab92547, 1:200; Abcam Inc.), N‐cadherin (ab18203, 1 ug/ml; Abcam Inc.), snail (1:1000, ab180714; Abcam), and twist (1:1000, ab49254; Abcam) were added for overnight incubation at 4°C. After washing with PBS three times, each time for 3 min, the biotin‐labeled secondary goat antirabbit antibody (HY90046, 1:100; Shanghai Hengyuan Biological Technology Co. Ltd., Shanghai, China) was added for incubation at 37°C for 30 min.
